Additional in vitro study examined anti-aging properties

As Sabinsa’s R&D Scientists expanded their understanding of probiotics, and developed new products based upon that increased knowledge, it was inevitable that published studies would soon follow. Sabinsa has recently published its first human clinical study on anti-acne benefits of LactoSporin®, the probiotic metabolite derived from the highly regarded probiotic LactoSpore®. A second published study, in vitro, demonstrated the anti-aging potential of this topical ingredient. Together, the research supports the use of LactoSporin for skin care applications.

“Novel Topical Application of a Postbiotic, LactoSporin, in Mild to Moderate Acne: A Randomized, Comparative Clinical Study to Evaluate its Efficacy, Tolerability and Safety” by Majeed M, Majeed S, Nagabhushanam K, Mundkur L, Rajalakshmi HR and Shah K was recently published in the journal Cosmetics (Cosmetics 2020; 7(3): 70). The study was conducted as a randomized, open-label, comparative study in 64 human volunteers with mild to moderate acne.


As one of the first systematic clinical studies of a probiotic metabolite, the research demonstrates the efficacy of LactoSporin 2% w/w cream in reducing the severity of acne in human volunteers, with the effects comparable to benzoyl peroxide 2.5% gel.

The anti-acne effects of LactoSporin were observed as early as three days. The three-week study measured improvement in skin condition based on dermatologist visual assessment and instrumental measurements of acne spots. A significant improvement in all the assessed parameters was observed in both LactoSporin and benzyl peroxide groups.

In addition, a significant decrease in sebaceous secretion was observed for LactoSporin, which resulted in reduced oiliness, pimples, acne spots and redness around the acne spot. Further, LactoSporin was found to be stable at an acidic pH and temperature range of 70 to 90°C, with antimicrobial activity against various pathogenic bacteria, including Cutibacterium acnes.

The study concluded that the efficacy of LactoSporin was equivalent to benzoyl peroxide in the treatment of mild-to-moderate acne lesions and has an advantage over benzoyl peroxide in individuals with oily skin.

“The emerging field of postbiotics continues to garner interest from the scientific community, and this is an area of immense interest to Sabinsa as well,” Shaheen Majeed, president Worldwide of Sabinsa and a co-author of the study. “While our room-temperature shelf-stable probiotic, LactoSpore (Bacillus coagulans MTCC 5856) has been successful in various applications ranging from supplements, beverages and baked goods, we wanted to explore the possibilities on the cosmetic front. Here we introduced, LactoSporin, a metabolic byproduct of our probiotic, to help improve the composition of the skin’s beneficial bacteria. This study clearly shows that LactoSporin is highly suitable for treating subjects with mild-to-moderate acne vulgaris.”

The cream was found to be safe and non-irritant in a repeated patch test conducted in 25 healthy individuals, prior to the anti-acne study.

Further details of this study may be accessed at

In the second study, “Skin Protective Activity of LactoSporin – the Extracellular Metabolite from Bacillus coagulans MTCC 5856” by Majeed M, Majeed S, Nagabhushanam K, Lawrence L, Arumugam S, and Mundkur L (Cosmetics 2020, 7(4), 76), LactoSporin was evaluated for its role in preserving skin integrity in cultured cells in vitro. LactoSporin was found to be a powerful antioxidant with reactive oxygen quenching activity. It showed protection against UV-induced cellular damage and inhibited collagenase activity, suggesting its potential use in preventing accelerated aging. LactoSporin was also found to inhibit the activity of the enzyme hyaluronidase, thereby preventing hyaluronic acid fragmentation, the major cause of skin moisture loss. Increased expression of transforming growth factor -β, and hyaluronan synthase in skin fibroblasts, suggests that LactoSporin could keep the skin moisturized and maintain skin firmness. The study concluded that LactoSporin is a skin protective postbiotic with wide application in cosmetic formulations.

“LactoSporin’s non-living microbial cell products possess several advantages over the probiotic itself, especially in skin care,” Majeed said. “It shows biological activity in a non-viable state, are easy to formulate, have favorable physiochemical properties and show improved inflammatory defense.”

Further details of this study may be accessed at